Light-scattering fingerprinting for characterization of smooth-muscle cell proliferation

Yang Liu; Josephine Allen; Young L. Kim; Vladimir Turzhitsky; Guillermo Ameer; Vadim Backman

doi:10.1117/12.529175

1 July 2004 Light-scattering fingerprinting for characterization of smooth-muscle cell proliferation

Yang Liu, Josephine Allen, Young L. Kim, Vladimir Turzhitsky, Guillermo Ameer, Vadim Backman

Proceedings Volume 5319, Laser Interaction with Tissue and Cells XV; (2004) https://doi.org/10.1117/12.529175
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Accelerated proliferation of smooth muscle cells (SMC) is known to play an integral role in atherosclerotic lesion formation. Thus, there has been significant interest in defining both positive and negative regulators of SMC growth. We have applied a novel optical technique referred to as four-dimensional light scattering fingerprinting (4D-ELF) that enables non-invasive assessment of living cells. 4D-ELF can serve for highly sensitive detection of slight alterations in cellular and subcellular microstructure. Using 4D-ELF, we characterized the proliferation of SMC grown on two different substrates: laminin and fibronectin. Fibronectin-grown SMC have been previously shown to be more proliferative. Our results indicate that light scattering can be used to monitor the changes in the intracellular structure caused by the cell-substrate interaction and differentiate between more and less proliferative SMCs. Thus, light scattering fingerprinting may potentially provide a quick, inexpensive, and accurate means to noninvasively characterize the proliferation of living cells as well as cell-biomaterial interaction.

Citation Download Citation

Yang Liu, Josephine Allen, Young L. Kim, Vladimir Turzhitsky, Guillermo Ameer, and Vadim Backman "Light-scattering fingerprinting for characterization of smooth-muscle cell proliferation", Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); https://doi.org/10.1117/12.529175

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KEYWORDS

Light scattering

Scattering

Tissues

Polarization

Principal component analysis

Transmission electron microscopy

Light wave propagation

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