Toward fluorescence detection of protein residues on surgical instruments

Patricia R. Richardson; Anita C. Jones; Robert L. Baxter; Helen C. Baxter; A. Gavin Whittaker; Gaynor A. Campbell

doi:10.1117/12.566604

9 June 2004 Toward fluorescence detection of protein residues on surgical instruments

Patricia R. Richardson, Anita C. Jones, Robert L. Baxter, Helen C. Baxter, A. Gavin Whittaker, Gaynor A. Campbell

Proceedings Volume 5502, Second European Workshop on Optical Fibre Sensors; (2004) https://doi.org/10.1117/12.566604
Event: Second European Workshop on Optical Fibre Sensors, 2004, Santander, Spain

Abstract

Prion proteins are the infectious agents that cause Creutzfeldt-Jakob Disease (CJD) in humans. These proteins are particularly resistant to normal sterilization procedures, and the theoretical risk of prion transmission via surgical instruments is of current public and professional concern. We are currently investigating fluorescence methods for the detection of proteins on surfaces, with a view to developing an optical-fiber-based system for routine, online monitoring of residual protein contamination on surgical instruments, in hospital sterilization departments. This paper presents preliminary results on the detection of femtomole amounts of fluorescently labelled protein on surgical steel and discusses some of the problems involved in the detection of fluorescence from metal samples.

Citation Download Citation

Patricia R. Richardson, Anita C. Jones, Robert L. Baxter, Helen C. Baxter, A. Gavin Whittaker, and Gaynor A. Campbell "Toward fluorescence detection of protein residues on surgical instruments", Proc. SPIE 5502, Second European Workshop on Optical Fibre Sensors, (9 June 2004); https://doi.org/10.1117/12.566604

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available