Application of the holographic interference microscope for investigation of ozone therapy influence on blood erythrocytes of patients <i>in vivo</i>

Tatyana V. Tishko; V. P. Titar; T. M. Barchotkina; D. N. Tishko

doi:10.1117/12.583399

24 September 2004 Application of the holographic interference microscope for investigation of ozone therapy influence on blood erythrocytes of patients in vivo

Tatyana V. Tishko, V. P. Titar, T. M. Barchotkina, D. N. Tishko

Author Affiliations +

Proceedings Volume 5582, Advanced Optoelectronics and Lasers; (2004) https://doi.org/10.1117/12.583399
Event: 2003 Chapter books, 2003, Bellingham, WA, United States

Abstract

The holographic methods of phase micro-objects visualization (the holographic phase contrast method and the method of holographic interferometry) are considered. Comparative analysis of classical and holographic methods in microscopy of phase micro-objects is carried out. An arrangement of the holographic interference microscope realizing the holographic methods and experimental results of 3-D imaging of native blood erythrocytes are presented. It is shown that 3-D morphology of blood erythrocytes reflects and determines the state of a human organism and those different physical and chemical factors and internal pathologies influence erythrocytes morphology. The holographic interference microscope was used for investigation of ozone therapy influence on human blood erythrocytes. Blood samples of 60 patients of different age with neurosensoric hardness of hearing before and after ozone therapy were investigated. It was shown that all patients have changed erythrocytes mrophology. Ozone therapy treatment results in normalization of erythrocytes morphology of patients.

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Tatyana V. Tishko, V. P. Titar, T. M. Barchotkina, and D. N. Tishko "Application of the holographic interference microscope for investigation of ozone therapy influence on blood erythrocytes of patients in vivo", Proc. SPIE 5582, Advanced Optoelectronics and Lasers, (24 September 2004); https://doi.org/10.1117/12.583399

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