Coherent light scattering by in vitro cell arrays observed with angle-resolved low-coherence interferometry

John W. Pyhtila; Adam Wax

doi:10.1117/12.592230

13 April 2005 Coherent light scattering by in vitro cell arrays observed with angle-resolved low-coherence interferometry

John W. Pyhtila, Adam Wax

Author Affiliations +

Proceedings Volume 5690, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX; (2005) https://doi.org/10.1117/12.592230
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Angle-resolved low coherence interferometry enables depth-resolved measurements of scattered light. The scattered light measurements can be used to recover structural information from sub-surface layers, such as the size of cell nuclei. Measurements of nuclear morphology, however, can be complicated by coherent scattering between adjacent cell nuclei. Previous studies have eliminated this component by applying a window filter to Fourier transformed data based upon the justification that the coherent scattering must necessarily occur over length scales greater than the cell nucleus size. To fully study this effect, we now present results of experiments designed to test the validity of this approach. We examine light scattered by regular cell arrays, created using stamped adhesive micro-patterned substrates. By varying the array spacing, the influence of cell-to-cell correlations on light scattering distributions is determined. The impact on nuclear morphology measurements within intact tissue samples is discussed.

Citation Download Citation

John W. Pyhtila and Adam Wax "Coherent light scattering by in vitro cell arrays observed with angle-resolved low-coherence interferometry", Proc. SPIE 5690, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, (13 April 2005); https://doi.org/10.1117/12.592230

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available