Asymmetry of rotational catalysis of single membrane-bound F0F1-ATP synthase

Nawid Zarrabi; Boris Zimmermann; Manuel Diez; Peter Graeber; Joerg Wrachtrup; Michael Boersch

doi:10.1117/12.597967

29 March 2005 Asymmetry of rotational catalysis of single membrane-bound F₀F₁-ATP synthase

Nawid Zarrabi, Boris Zimmermann, Manuel Diez, Peter Graeber, Joerg Wrachtrup, Michael Boersch

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.597967
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Synthesis of the cellular 'energy currency' ATP is catalyzed by membrane-bound F₀F₁-ATP synthases. The chemical reaction at three binding sites in the F₁ part is coupled to proton translocation through the membrane-integrated F₀ part by an internal rotation of subunits. We examined the rotary movements of the ε-subunit of the 'rotor' with respect to the b-subunits of the 'stator' by single-molecule fluorescence resonance energy transfer (FRET). Rotation of ε during ATP hydrolysis is divided into three major steps with constant FRET level corresponding to three binding sites. Different catalytic activities of the individual binding sites were observed depending on the relative orientation of the 'rotor'. Computer simulations of the FRET signals and non-equally distributed orientations of ε strongly corroborate asymmetry of catalysis in F₀F₁-ATP synthase.

Citation Download Citation

Nawid Zarrabi, Boris Zimmermann, Manuel Diez, Peter Graeber, Joerg Wrachtrup, and Michael Boersch "Asymmetry of rotational catalysis of single membrane-bound F₀F₁-ATP synthase", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.597967

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available