Attachment of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes to beef and inactivation using hydrodynamic pressure processing

Jitendra R. Patel; Morse B. Solomon

doi:10.1117/12.630569

8 November 2005 Attachment of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes to beef and inactivation using hydrodynamic pressure processing

Jitendra R. Patel, Morse B. Solomon

Author Affiliations +

Proceedings Volume 5996, Optical Sensors and Sensing Systems for Natural Resources and Food Safety and Quality; 59961E (2005) https://doi.org/10.1117/12.630569
Event: Optics East 2005, 2005, Boston, MA, United States

Abstract

The attachment strength of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes to beef surface was evaluated. The effect of bacterial attachment strength on inactivation by HDP treatment was studied. Bacterial attachment was defined as loosely attached (analyzed first by running diluent over the beef cubes) and strongly attached (analyzed again rinsed cubes by pummeling for 2 min in diluent) to the beef surface. Most attachment of these three pathogens occurred within 10 min. There was no significant difference in attachment strength of pathogens during the first 30 minutes exposure time. Strongly attached bacteria to beef surface were inactivated by HDP at a greater rate and the reduction was significant for all three bacteria (0.52, 0.37, and 0.43 log₁₀ CFU/g for E. coli O157:H7, S. Typhimurium, and L. monocytogenes, respectively). No clear indications were obtained for sub-lethal damage of microorganisms surviving the HDP treatment. These findings disclose that HDP treatment is more lethal to pathogenic bacteria strongly attached to beef surface compared to those planktonic or loosely attached cells.

Citation Download Citation

Jitendra R. Patel and Morse B. Solomon "Attachment of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes to beef and inactivation using hydrodynamic pressure processing", Proc. SPIE 5996, Optical Sensors and Sensing Systems for Natural Resources and Food Safety and Quality, 59961E (8 November 2005); https://doi.org/10.1117/12.630569

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available