A fluorescent bio-aerosol point detector incorporating excitation, emission, and lifetime data

Pierre C. Trepagnier; Robert F. Dillon; Philip D. Henshaw; David P. McCampbell

doi:10.1117/12.684754

17 October 2006 A fluorescent bio-aerosol point detector incorporating excitation, emission, and lifetime data

Pierre C. Trepagnier, Robert F. Dillon, Philip D. Henshaw, David P. McCampbell

Proceedings Volume 6377, Advanced Environmental, Chemical, and Biological Sensing Technologies IV; 637708 (2006) https://doi.org/10.1117/12.684754
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

We present a novel approach to a biological point detector system: extracting maximal information from fluorescence by using as much of the full excitation-emission-lifetime (XML) fluorescence space as can be conveniently gathered. Our paper has two parts. In the first part, we present initial XML spectral data gathered under Phase I of the DARPA Spectral-Sensing of Bio-Aerosols (SSBA) program using a commercial laboratory spectrofluorometer and illustrate its analysis in a multi-dimensional Principal Components Analysis (PCA) data space. We demonstrate classification using the spectral angle (SA) methodology developed for hyperspectral imaging in this PCA hyperspace. In the second part, we present a design for a custom trigger sensor developed in Phase II of the DARPA program. This Phase II sensor was motivated by the Phase I results and is intended to exploit them by gathering XML data at a rate consistent with near-real-time triggering.

Citation Download Citation

Pierre C. Trepagnier, Robert F. Dillon, Philip D. Henshaw, and David P. McCampbell "A fluorescent bio-aerosol point detector incorporating excitation, emission, and lifetime data", Proc. SPIE 6377, Advanced Environmental, Chemical, and Biological Sensing Technologies IV, 637708 (17 October 2006); https://doi.org/10.1117/12.684754

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