Switchable surface coatings for control over protein adsorption

Martin A. Cole; Marek Jasieniak; Nicolas H. Voelcker; Helmut Thissen; Roger Horn; Hans J. Griesser

doi:10.1117/12.696290

14 December 2006 Switchable surface coatings for control over protein adsorption

Martin A. Cole, Marek Jasieniak, Nicolas H. Voelcker, Helmut Thissen, Roger Horn, Hans J. Griesser

Proceedings Volume 6416, Biomedical Applications of Micro- and Nanoengineering III; 641606 (2006) https://doi.org/10.1117/12.696290
Event: SPIE Smart Materials, Nano- and Micro-Smart Systems, 2006, Adelaide, Australia

Abstract

Control over biomolecule interactions at interfaces is becoming an increasingly important goal for a range of scientific fields and is being intensively studied in areas of biotechnological, biomedical and materials science. Improvement in the control over materials and biomolecules is particularly important to applications such as arrays, biosensors, tissue engineering, drug delivery and 'lab on a chip' devices. Further development of these devices is expected to be achieved with thin coatings of stimuli responsive materials that can have their chemical properties 'switched' or tuned to stimulate a certain biological response such as adsorption/desorption of proteins. Switchable coatings show great potential for the realisation of spatial and temporal immobilisation of cells and biomolecules such as DNA and proteins. This study focuses on protein adsorption onto coatings of the thermosensitive polymer poly(N-isopropylacrylamide) (pNIPAM) which can exhibit low and high protein adsorption properties based on its temperature dependent conformation. At temperatures above its lower critical solution temperature (LCST) pNIPAM polymer chains are collapsed and protein adsorbing whilst below the LCST they are hydrated and protein repellent. Coatings of pNIPAM on silicon wafers were prepared by free radical polymerisation in the presence of surface bound polymerisable groups. Surface analysis and protein adsorption was carried out using X-ray photoelectron spectroscopy, time of flight secondary ion mass spectrometry and contact angle measurements. This study is expected to aid the development of stimuli-responsive coatings for biochips and biodevices.

Citation Download Citation

Martin A. Cole, Marek Jasieniak, Nicolas H. Voelcker, Helmut Thissen, Roger Horn, and Hans J. Griesser "Switchable surface coatings for control over protein adsorption", Proc. SPIE 6416, Biomedical Applications of Micro- and Nanoengineering III, 641606 (14 December 2006); https://doi.org/10.1117/12.696290

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