Axially resolved cell imaging by intensity modulated total internal reflection fluorescence microscopy (IM-TIRFM)

Herbert Schneckenburger; Harald Baumann; Michael Wagner; Wolfgang S. L. Strauss

doi:10.1117/12.699826

19 February 2007 Axially resolved cell imaging by intensity modulated total internal reflection fluorescence microscopy (IM-TIRFM)

Herbert Schneckenburger, Harald Baumann, Michael Wagner, Wolfgang S. L. Strauss

Author Affiliations +

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64411E (2007) https://doi.org/10.1117/12.699826
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Intensity modulation was introduced to total internal reflection fluorescence microscopy (TIRFM). This permits to obtain information about the axial distribution of fluorescent dyes in close vicinity to the surface of adherent cells and allows the application of (commercial) objective based TIRFM systems at a fixed angle of illumination. However, this new method seems to be less suitable for quantitative measurements of cell-substrate topology as compared to variableangle prism based TIRFM.

Citation Download Citation

Herbert Schneckenburger, Harald Baumann, Michael Wagner, and Wolfgang S. L. Strauss "Axially resolved cell imaging by intensity modulated total internal reflection fluorescence microscopy (IM-TIRFM)", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411E (19 February 2007); https://doi.org/10.1117/12.699826

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