Assessment of microcirculation frequency components through functional diffuse reflectance spectroscopy: a promising tool for noninvasive skin diagnosis

Paulo R. Bargo; InSeok Seo; Nikiforos Kollias

doi:10.1117/12.848217

2 March 2010 Assessment of microcirculation frequency components through functional diffuse reflectance spectroscopy: a promising tool for noninvasive skin diagnosis

Paulo R. Bargo, InSeok Seo, Nikiforos Kollias

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Proceedings Volume 7548, Photonic Therapeutics and Diagnostics VI; 75480O (2010) https://doi.org/10.1117/12.848217
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Measurement of cutaneous microcirculation is of great importance for clinical evaluations as many biological processes (i.e. inflammation) activate superficial vessels. Diffuse Reflectance Spectroscopy (DRS) is a widely used method to assess cutaneous microcirculation since it is based on the spectral characteristics of skin chromophores, particularly the strong absorption bands of blood in the visible spectral range. However, the DRS system only provides a snapshot of the blood content in tissue from the reflectance detected at a single exposure time, and it fails to demonstrate dynamic changes of blood flow inside the tissues. In the present study, a prototype system for functional DRS (fDRS) has been developed for the sequential acquisition of multiple reflectance spectra in the visible range at sub-second intervals. Twelve healthy subjects with skin phototype I-III were recruited for a UV skin phototest where the subject's back was exposed to an increasing dose of solar-simulated radiation at a maximum of 3 minimum erythema dose (MED). Evaluations included conventional DRS, functional DRS, and a laser Doppler flowmeter/imaging system. In a frequency analysis results, there were two distinctive frequency components. A low-frequency component was found near 0.03-0.1 Hz, and another high-frequency component near 0.9-1.2 Hz which is synchronous with heart pulsations. The magnitude of the high-frequency and the steady-state components of the fDRS signal increased with increase in exposure dose. These results demonstrate the potential of this technique for noninvasive assessment of cutaneous microcirculation.

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Paulo R. Bargo, InSeok Seo, and Nikiforos Kollias "Assessment of microcirculation frequency components through functional diffuse reflectance spectroscopy: a promising tool for noninvasive skin diagnosis", Proc. SPIE 7548, Photonic Therapeutics and Diagnostics VI, 75480O (2 March 2010); https://doi.org/10.1117/12.848217

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KEYWORDS

Skin

Absorbance

Blood circulation

Blood

Diffuse reflectance spectroscopy

Reflectivity

Tissues

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