Combining optoacoustics and resonance Raman spectroscopy for quantification of biomolecules in situ

Merve Meinhardt-Wollweber

doi:10.1117/12.906646

1 February 2012 Combining optoacoustics and resonance Raman spectroscopy for quantification of biomolecules in situ

Merve Meinhardt-Wollweber

Proceedings Volume 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry; 821902 (2012) https://doi.org/10.1117/12.906646
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Resonant Raman spectroscopy is a technique to select and enhance the vibrational fingerprint of target molecules. Amplification of a specific Raman spectrum or specific components therein may be by several orders of magnitude. This allows to detect a molecular fingerprint even at low concentrations or in fluorescent environment without external signal enhancement. We use this approach to identify carotenoids of the antioxidant network. Quantification of the carotenoids relies on knowledge about attenuation of excitation light and Raman signal on the way through a given sample. To calculate this attenuation, the optical properties of the sample have to be known. Optoacoustics is a hybrid technique for non-invasive measurement of optical properties. We present a combined fiber sensor for optoacoustics and Raman spectroscopy allowing to probe optical properties parallel to Raman measurements. To the best of our knowledge, this is the first time that optoacoustics and vibrational spectroscopy have been combined in one sensor. It paves the way for identification and quantification of Raman active molecules in the living tissue.

Citation Download Citation

Merve Meinhardt-Wollweber "Combining optoacoustics and resonance Raman spectroscopy for quantification of biomolecules in situ", Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 821902 (1 February 2012); https://doi.org/10.1117/12.906646

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KEYWORDS

Raman spectroscopy

Molecules

Signal attenuation

Optical properties

Absorption

Sensors

Skin

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