Singlet oxygen induced advanced glycation end-product photobleaching of in vivo human fingertip autofluorescence

Bin Deng; Anabel Simental; Patrick Lutz; George Shaheen; Joseph Chaiken

doi:10.1117/12.914050

1 February 2012 Singlet oxygen induced advanced glycation end-product photobleaching of in vivo human fingertip autofluorescence

Bin Deng, Anabel Simental, Patrick Lutz, George Shaheen, Joseph Chaiken

Author Affiliations +

Proceedings Volume 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry; 82190D (2012) https://doi.org/10.1117/12.914050
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Nonenzymatic glycation and oxidation of ubiquitous proteins in vivo leads to irreversible formation of advanced glycation end products (AGEs). Due to their relatively long half life and low clearance rate AGEs tend to accumulate within static tissues and the circulatory system. Spectra obtained using 830 nm near-infrared (NIR) excitation suggest that the so-called "autofluorescence" from all tissues has a finite number of sources but the fact that senior and diabetic subjects produce more than other members of the general population suggests that a significant portion of the total autofluorescence from all sources originates from AGEs. Using pentosidine generated in a reaction mixture as described by Monnier as representative, an in vitro study unveiled very similar fluorescence and photobleaching pattern as observed for autofluorescence in vivo. A series of oxygen, air and argon purging experiments on the pentosidine-generating reaction mixture suggests that pentosidine is a singlet oxygen sensitizer and secondary reactions between the pentosidine itself and/or other fluorophores and the photosensitized singlet oxygen explain the observed photobleaching. Ab initio Gaussian calculations on pentosidine reveal the existence of low-lying triplet excited states required for the sensitization of ground state oxygen. A commercially available product known as singlet oxygen sensor green (SOSG) that specifically serves as a singlet oxygen detection reagent confirms the generation of singlet oxygen from NIR irradiated pentosidine trimixture. This study provides one definite chemical mechanism for understanding in vivo human skin autofluorescence and photobleaching.

Citation Download Citation

Bin Deng, Anabel Simental, Patrick Lutz, George Shaheen, and Joseph Chaiken "Singlet oxygen induced advanced glycation end-product photobleaching of in vivo human fingertip autofluorescence", Proc. SPIE 8219, Biomedical Vibrational Spectroscopy V: Advances in Research and Industry, 82190D (1 February 2012); https://doi.org/10.1117/12.914050

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