Spectroscopy Of Nitrenes Bound To a-Chymotrypsin

Matthew S. Platz; K. Kanakarajan; R. P. Goodrich; M. J. T. Young; S. Soundararajan

doi:10.1117/12.942690

19 February 1988 Spectroscopy Of Nitrenes Bound To ∝-Chymotrypsin

Matthew S. Platz, K. Kanakarajan, R. P. Goodrich, M. J. T. Young, S. Soundararajan

Proceedings Volume 0847, New Directions in Photodynamic Therapy; (1988) https://doi.org/10.1117/12.942690
Event: Cambridge Symposium on Optics in Medicine and Visual Image Processing, 1987, San Diego, CA, United States

Abstract

Reaction of ∝-chymotrypsin with various aryl azido acylimidazoles leads to covalent attachment of the aryl azide photoaffinity label. Photolysis of the polycrystalline enzyme or a frozen solution of the enzyme at 77K leads to intense EPR signals of the triplet nitrenes. The position of the EPR resonance fields and the decay kinetics can be interpreted in terms of binding of the label within or outside the binding pocket of the enzyme.

Citation Download Citation

Matthew S. Platz, K. Kanakarajan, R. P. Goodrich, M. J. T. Young, and S. Soundararajan "Spectroscopy Of Nitrenes Bound To ∝-Chymotrypsin", Proc. SPIE 0847, New Directions in Photodynamic Therapy, (19 February 1988); https://doi.org/10.1117/12.942690

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