Biomimetic approaches for engineered organ chips and skin electronics for in vitro diagnostics

Kahp-Yang Suh; Changhyun Pang; Kyung-Jin Jang; Hong Nam Kim; Alex Jiao; Nathaniel S. Hwang; Min Sung Kim; Do-Hyun Kang; Deok-Ho Kim

doi:10.1117/12.2000264

24 October 2012 Biomimetic approaches for engineered organ chips and skin electronics for in vitro diagnostics

Kahp-Yang Suh, Changhyun Pang, Kyung-Jin Jang, Hong Nam Kim, Alex Jiao, Nathaniel S. Hwang, Min Sung Kim, Do-Hyun Kang, Deok-Ho Kim

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Proceedings Volume 8548, Nanosystems in Engineering and Medicine; 854831 (2012) https://doi.org/10.1117/12.2000264
Event: SPIE Nanosystems in Engineering + Medicine, 2012, Incheon, Korea, Republic of

Abstract

Two kinds of biomimetic systems including engineered organ chip and flexible electronic sensor are presented. First, in vivo, renal tubular epithelial cells are exposed to luminal fluid shear stress (FSS) and a transepithelial osmotic gradient. In this study, we used a simple collecting-duct-on-a-chip to investigate the role of an altered luminal microenvironment in the translocation of aquaporin-2 (AQP2) and the reorganization of actin cytoskeleton (F-actin) in primary cultured inner medullary collecting duct (IMCD) cells of rat kidney. We demonstrate that several factors (i.e., luminal FSS, hormonal stimulation, transepithelial osmotic gradient) collectively exert a profound effect on the AQP2 trafficking in the collecting ducts, which is associated with actin cytoskeletal reorganization. Furthermore, with this kidney-mimicking chip, renal toxicity of cisplatin was tested under static and fluidic conditions, suggesting the physiological relevancy of fluidic environment compared to static culture. Second, we present a simple architecture for a flexible and highly sensitive strain sensor that enables the detection of pressure, shear and torsion. The device is based on two interlocked arrays of high-aspect-ratio Pt-coated polymeric nanofibres that are supported on thin polydimethylsiloxane layers. When different sensing stimuli are applied, the degree of interconnection and the electrical resistance of the sensor changes in a reversible, directional manner with specific, discernible strain-gauge factors. We show that the sensor can be used to monitor signals ranging from human heartbeats to the impact of a bouncing water droplet on a superhydrophobic surface.

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Kahp-Yang Suh, Changhyun Pang, Kyung-Jin Jang, Hong Nam Kim, Alex Jiao, Nathaniel S. Hwang, Min Sung Kim, Do-Hyun Kang, and Deok-Ho Kim "Biomimetic approaches for engineered organ chips and skin electronics for in vitro diagnostics", Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 854831 (24 October 2012); https://doi.org/10.1117/12.2000264

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KEYWORDS

Sensors

Microfluidics

Polymers

In vitro testing

Biomimetics

Electronics

Kidney

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