Noninvasive imaging of absolute PpIX concentration distribution in
nonmelanoma skin tumors at pre-PDT

Ulas Sunar; Daniel Rohrbach; Janet Morgan; Natalie Zeitouni

doi:10.1117/12.2005078

13 March 2013 Noninvasive imaging of absolute PpIX concentration distribution in nonmelanoma skin tumors at pre-PDT

Ulas Sunar, Daniel Rohrbach, Janet Morgan, Natalie Zeitouni

Proceedings Volume 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII; 85680C (2013) https://doi.org/10.1117/12.2005078
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Photodynamic Therapy (PDT) has proven to be an effective treatment option for nonmelanoma skin cancers. The ability to quantify the concentration of drug in the treated area is crucial for effective treatment planning as well as predicting outcomes. We utilized spatial frequency domain imaging for quantifying the accurate concentration of protoporphyrin IX (PpIX) in phantoms and in vivo. We correct fluorescence against the effects of native tissue absorption and scattering parameters. First we quantified the absorption and scattering of the tissue non-invasively. Then, we corrected raw fluorescence signal by compensating for optical properties to get the absolute drug concentration. After phantom experiments, we used basal cell carcinoma (BCC) model in Gli mice to determine optical properties and drug concentration in vivo at pre-PDT.

Citation Download Citation

Ulas Sunar, Daniel Rohrbach, Janet Morgan, and Natalie Zeitouni "Noninvasive imaging of absolute PpIX concentration distribution in nonmelanoma skin tumors at pre-PDT", Proc. SPIE 8568, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXII, 85680C (13 March 2013); https://doi.org/10.1117/12.2005078

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available