Preparation of ferrous chelate of hairtail (Trichiurus haumela) protein hydrolysate (Fe(II)-HPH) and its antibacterial activity

Huimin Lin; Bin Zhang; Tian Yu; Shanggui Deng

doi:10.1117/12.2019704

19 March 2013 Preparation of ferrous chelate of hairtail (Trichiurus haumela) protein hydrolysate (Fe(II)-HPH) and its antibacterial activity

Huimin Lin, Bin Zhang, Tian Yu, Shanggui Deng

Author Affiliations +

Proceedings Volume 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering; 876212 (2013) https://doi.org/10.1117/12.2019704
Event: Third International Conference on Photonics and Image in Agriculture Engineering (PIAGENG 2013), 2013, Sanya, China

Abstract

The preparation of a ferrous chelate of hairtail (Trichiurus haumela) protein hydrolysate (Fe(II)-HPH) and its antibacterial activity were studied. The optimal conditions of hydrolysis by papain and ferrous chelation were obtained by single-factor experiments and orthogonal test, with the antibacterial activities as the index. In addition, the antibacterial activity of Fe(II)-HPH was evaluated using the Plackett–Burman design. The orthogonal test results showed that Fe(II)-HPH had an antibacterial activity of 98.3% under a temperature of 40 °C at pH 6.5 for an enzymolysis duration of eight hours in the presence of 20,000 U/g of enzyme. The Plackett–Burman design analysis showed that the three most significant factors (P < 0.05) influencing the antibacterial activity of Fe(II)-HPH were pH, the concentration (mg/mL), and presence of magnesium sulfate.

Citation Download Citation

Huimin Lin, Bin Zhang, Tian Yu, and Shanggui Deng "Preparation of ferrous chelate of hairtail (Trichiurus haumela) protein hydrolysate (Fe(II)-HPH) and its antibacterial activity", Proc. SPIE 8762, PIAGENG 2013: Intelligent Information, Control, and Communication Technology for Agricultural Engineering, 876212 (19 March 2013); https://doi.org/10.1117/12.2019704

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