Fluorescence interference contrast based approach to study real time interaction of melittin with plasma membranes

Sharad Gupta; Dong Gui; Roya Zandi; Sarjeet Gill; Umar Mohideen

doi:10.1117/12.2038483

4 March 2014 Fluorescence interference contrast based approach to study real time interaction of melittin with plasma membranes

Sharad Gupta, Dong Gui, Roya Zandi, Sarjeet Gill, Umar Mohideen

Author Affiliations +

Proceedings Volume 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII; 894723 (2014) https://doi.org/10.1117/12.2038483
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Melittin is an anti-bacterial and hemolytic toxic peptide found in bee venom. Cell lysis behavior of peptides has been widely investigated, but the exact interaction mechanism of lytic peptides with lipid membranes and its constituents has not been understood completely. In this paper we study the melittin interaction with lipid plasma membranes in real time using non-invasive and non-contact fluorescence interference contrast microscopy (FLIC). Particularly the interaction of melittin with plasma membranes was studied in a controlled molecular environment, where these plasma membrane were composed of saturated lipid, 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and unsaturated lipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine(DOPC) with and without cholesterol. We found out that melittin starts to form nanometer size pores in the plasma membranes shortly after interacting with membranes. But the addition of cholesterol in plasma membrane slows down the pore formation process. Our results show that inclusion of cholesterol to the plasma membranes make them more resilient towards pore formation and lysis of membrane.

Citation Download Citation

Sharad Gupta, Dong Gui, Roya Zandi, Sarjeet Gill, and Umar Mohideen "Fluorescence interference contrast based approach to study real time interaction of melittin with plasma membranes", Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 894723 (4 March 2014); https://doi.org/10.1117/12.2038483

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available