Detection of squamous carcinoma cells using gold nanoparticles

Wei-Yun Dai; Sze-tsen Lee; Yih-Chih Hsu

doi:10.1117/12.2081783

9 March 2015 Detection of squamous carcinoma cells using gold nanoparticles

Wei-Yun Dai, Sze-tsen Lee, Yih-Chih Hsu

Proceedings Volume 9324, Biophotonics and Immune Responses X; 93240N (2015) https://doi.org/10.1117/12.2081783
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

The goal of this study is to use gold nanoparticle as a diagnostic agent to detect human squamous carcinoma cells. Gold nanoparticles were synthesized and the gold nanoparticle size was 34.3 ± 6.2 nm. Based on the over-expression of epidermal growth factor receptor (EGFR) biomarkers in squamous carcinoma cells, we hypothesized that EGFR could be a feasible biomarker with a target moiety for detection. We further modified polyclonal antibodies of EGFR on the surface of gold nanoparticles. We found selected squamous carcinoma cells can be selectively detected using EGFR antibody-modified gold nanoparticles via receptor-mediated endocytosis. Cell death was also examined to determine the survival status of squamous carcinoma cells with respect to gold nanoparticle treatment and EGFR polyclonal antibody modification.

Citation Download Citation

Wei-Yun Dai, Sze-tsen Lee, and Yih-Chih Hsu "Detection of squamous carcinoma cells using gold nanoparticles", Proc. SPIE 9324, Biophotonics and Immune Responses X, 93240N (9 March 2015); https://doi.org/10.1117/12.2081783

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