Comparison of the polarization properties in the retinas of different rodents using high resolution polarization sensitive OCT

Stanislava Fialová; Marco Augustin; Roberto Plasenzotti; Sabine Rauscher; Marion Gröger; Michael Pircher; Christoph K. Hitzenberger; Bernhard Baumann

doi:10.1117/12.2183449

9 July 2015 Comparison of the polarization properties in the retinas of different rodents using high resolution polarization sensitive OCT

Stanislava Fialová, Marco Augustin, Roberto Plasenzotti, Sabine Rauscher, Marion Gröger, Michael Pircher, Christoph K. Hitzenberger, Bernhard Baumann

Author Affiliations +

Proceedings Volume 9541, Optical Coherence Imaging Techniques and Imaging in Scattering Media; 95410P (2015) https://doi.org/10.1117/12.2183449
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Animal models play an important role for understanding the pathophysiology of glaucoma and age-related macular degeneration. With these models, longitudinal studies can be performed and therefore there is need for non-invasive evaluation of disease progress. For that purpose optical coherence tomography (OCT) can be used. Since tissues with different polarization properties are important in these diseases, polarization sensitive OCT (PS-OCT) could be a valuable tool in preclinical research. In this work a high resolution PS-OCT (HR-PS-OCT) system was used in-vivo for rodent retinal imaging. A super luminescent diode with a bandwidth of 100 nm was used as a light source that yielded an axial resolution of 5.1 μm in air (3.8 μm in tissue). The A-scan rate was 83 kHz, a whole 3D dataset was acquired in a few seconds (1536x1024x200 pixels in 3.5 s) which reduced motion artifacts. Rats (Sprague-Dawley, Long-Evans and Brown Norway) as well as mice (C57BL/6) were imaged. High resolution reflectivity images showed all retinal layers in all animals. From acquired data also phase retardation, fast axis orientation and degree of polarization uniformity (DOPU) images were calculated. On phase retardation images sclera was identified as birefringent and retinal pigment epithelium (RPE) and choroid as depolarizing tissues. Our results demonstrate the suitability of the system for high speed/resolution imaging in follow up studies on rodents.

Citation Download Citation

Stanislava Fialová, Marco Augustin, Roberto Plasenzotti, Sabine Rauscher, Marion Gröger, Michael Pircher, Christoph K. Hitzenberger, and Bernhard Baumann "Comparison of the polarization properties in the retinas of different rodents using high resolution polarization sensitive OCT", Proc. SPIE 9541, Optical Coherence Imaging Techniques and Imaging in Scattering Media, 95410P (9 July 2015); https://doi.org/10.1117/12.2183449

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Polarization

Tissues

Optical coherence tomography

Imaging systems

Sclera

Image resolution

Reflectivity

Show All Keywords

Keywords/Phrases

Search In:

Publication Years