Online monitoring of mechanical properties of three-dimensional tissue engineered constructs for quality assessment

Yvonne Reinwald; Pierre O. Bagnaninchi; Ying Yang; Yanny M. Baba Ismail; Alicia J. El Haj

doi:10.1117/12.2212320

9 March 2016 Online monitoring of mechanical properties of three-dimensional tissue engineered constructs for quality assessment

Yvonne Reinwald, Pierre O. Bagnaninchi, Ying Yang, Yanny M. Baba Ismail, Alicia J. El Haj

Author Affiliations +

Proceedings Volume 9710, Optical Elastography and Tissue Biomechanics III; 971007 (2016) https://doi.org/10.1117/12.2212320
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Mechanical preconditioning and mechanical properties of tissue engineered constructs are essential for their capability to regenerate damaged tissues. To online monitor the mechanical properties a hydrostatic pressure bioreactor was coupled with optical coherence tomography into a new image modality termed hydrostatic pressure optical coherence elastography (HP-OCE). HP-OCE was utilised to assess the properties of three-dimensional (3D) tissue constructs while being physically stimulated within the hydrostatic force bioreactor. Hydrogels have been infiltrated into porous rapid prototyped or salt-leached scaffolds to mimic heterogeneous mechanical properties of cell-seeded constructs. Variations of mechanical properties in the solid scaffolds and agarose gels with different gel concentrations as well as the presences of cells have been clearly delineated by HP-OCE. Results indicate that HP-OCE allows contactless real-time non-invasive monitoring of the mechanical properties of tissue constructs and the effect of physical stimulation on cellular activities.

Citation Download Citation

Yvonne Reinwald, Pierre O. Bagnaninchi, Ying Yang, Yanny M. Baba Ismail, and Alicia J. El Haj "Online monitoring of mechanical properties of three-dimensional tissue engineered constructs for quality assessment", Proc. SPIE 9710, Optical Elastography and Tissue Biomechanics III, 971007 (9 March 2016); https://doi.org/10.1117/12.2212320

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KEYWORDS

Tissues

Optical coherence tomography

Elastography

Tissue optics

Coherence (optics)

Interfaces

Solids

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