4D phase-space multiplexing for fluorescent microscopy

Hsiou-Yuan Liu; Jingshan Zhong; Laura Waller

doi:10.1117/12.2213610

24 March 2016 4D phase-space multiplexing for fluorescent microscopy

Hsiou-Yuan Liu, Jingshan Zhong, Laura Waller

Proceedings Volume 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management; 97200A (2016) https://doi.org/10.1117/12.2213610
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Phase-space measurements enable characterization of second-order spatial coherence properties and can be used for digital aberration removal or 3D position reconstruction. Previous methods use a scanning aperture to measure the phase space spectrogram, which is slow and light inefficient, while also attenuating information about higher-order correlations. We demonstrate a significant improvement of speed and light throughput by incorporating multiplexing techniques into our phase-space imaging system. The scheme implements 2D coded aperture patterning in the Fourier (pupil) plane of a microscope using a Spatial Light Modulator (SLM), while capturing multiple intensity images in real space. We compare various multiplexing schemes to scanning apertures and show that our phase-space reconstructions are accurate for experimental data with biological samples containing many 3D fluorophores.

Conference Presentation

Citation Download Citation

Hsiou-Yuan Liu, Jingshan Zhong, and Laura Waller "4D phase-space multiplexing for fluorescent microscopy", Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200A (24 March 2016); https://doi.org/10.1117/12.2213610

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