A label-free optical biosensor for serotyping "unknown" influenza viruses

Hanyuan Zhang; Carole Henry Dunand; Patrick Wilson; Benjamin L. Miller

doi:10.1117/12.2228245

12 May 2016 A label-free optical biosensor for serotyping "unknown" influenza viruses

Hanyuan Zhang, Carole Henry Dunand, Patrick Wilson, Benjamin L. Miller

Proceedings Volume 9824, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVII; 982405 (2016) https://doi.org/10.1117/12.2228245
Event: SPIE Defense + Security, 2016, Baltimore, MD, United States

Abstract

The ability to accurately classify influenza viruses is critical to understanding patterns of infection, vaccine efficacy, and to the process of developing new vaccines. Unfortunately, this task is hampered both by the virus’ ability to undergo antigenic drift and shift (rendering it a “previously unknown” strain), and by technological limitations. In an effort to overcome these challenges, we have developed a label-free human monoclonal antibody array for flu serology, using a pattern recognition approach to assign virus serotype. The array is built on the Arrayed Imaging Reflectometry (AIR) platform. AIR relies on the creation of a near-perfect antireflective condition on the surface of a silicon chip. When this antireflective condition is perturbed because of binding to an antibody spot (or other immobilized probe molecule), binding may be sensitively and quantitatively detected as an increase in reflected light. We describe fabrication and characterization of the array, and preliminary testing with isolated influenza hemagglutinin. We anticipate that this approach may be extended to other viruses by expansion of the array.

Citation Download Citation

Hanyuan Zhang, Carole Henry Dunand, Patrick Wilson, and Benjamin L. Miller "A label-free optical biosensor for serotyping "unknown" influenza viruses", Proc. SPIE 9824, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVII, 982405 (12 May 2016); https://doi.org/10.1117/12.2228245

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