Quantitative evaluation of dynamic activity of HeLa cells using dynamic full-field optical coherence microscopy: viable and dead cells

Soongho Park; Thien Nguyen; Amir Gandjbakhche

doi:10.1117/12.2610456

7 March 2022 Quantitative evaluation of dynamic activity of HeLa cells using dynamic full-field optical coherence microscopy: viable and dead cells

Soongho Park, Thien Nguyen, Amir Gandjbakhche

Author Affiliations +

Proceedings Volume PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX; PC119640I (2022) https://doi.org/10.1117/12.2610456
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79±0.5 Hz and 2.44±1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57±0.71 Hz, whereas the magnitude was significantly decreased to 0.53±0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods.

Conference Presentation

Citation Download Citation

Soongho Park, Thien Nguyen, and Amir Gandjbakhche "Quantitative evaluation of dynamic activity of HeLa cells using dynamic full-field optical coherence microscopy: viable and dead cells", Proc. SPIE PC11964, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XX, PC119640I (7 March 2022); https://doi.org/10.1117/12.2610456

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