Label-free multiphoton characterization of mouse brain cortical structures

Andrew T. Francis; Bryce Manifold; Elena C. Carlson; Ruoqian Hu; Andrew H. Hill; Shuaiqian Men; Dan Fu

doi:10.1117/12.2608995

2 March 2022 Label-free multiphoton characterization of mouse brain cortical structures

Andrew T. Francis, Bryce Manifold, Elena C. Carlson, Ruoqian Hu, Andrew H. Hill, Shuaiqian Men, Dan Fu

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730A (2022) https://doi.org/10.1117/12.2608995
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

In recent studies, stimulated Raman scattering (SRS) and transient absorption microscopy (TAM) have been employed for label-free mapping of biomolecules (e.g., proteins and lipids) in brain tissues and hemoglobin in red blood cells, respectively. In this study, we combined SRS and TAM to simultaneously image cell densities and capillary structure in vivo at the highest reported imaging depth, ~300 µm, for either technique. This multimodal approach resulted in label-free identification of endothelial cells and pericytes in vivo with 90% accuracy using a machine learning classifier. Simultaneous two-photon excited fluorescence microscopy serving as the ground truth.

Conference Presentation

Citation Download Citation

Andrew T. Francis, Bryce Manifold, Elena C. Carlson, Ruoqian Hu, Andrew H. Hill, Shuaiqian Men, and Dan Fu "Label-free multiphoton characterization of mouse brain cortical structures", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730A (2 March 2022); https://doi.org/10.1117/12.2608995

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