Quantification and improvement of signal-to-background ratio of endogenous fluorescence imaging using double-clad fiber endoscopes

Eric Brace; Pierre Lane

doi:10.1117/12.2650518

17 March 2023 Quantification and improvement of signal-to-background ratio of endogenous fluorescence imaging using double-clad fiber endoscopes

Eric Brace, Pierre Lane

Author Affiliations +

Proceedings Volume PC12356, Endoscopic Microscopy XVIII; PC123560N (2023) https://doi.org/10.1117/12.2650518
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Optical coherence tomography combined with autofluorescence imaging (OCT-AFI) has shown potential for cancer detection. However, signal-to-background ratio (SBR) of the fluorescence modality is limited by low endogenous fluorophore signal, which is often similar in magnitude to system background fluorescence. We propose a double-clad fiber endoscope using time-resolved detection and index matching fluids at high reflection interfaces to improve SBR such that low-signal fluorophores can be visualized. Zemax and experimental measurements are used to assess device performance. Replacing air gaps with index matching fluid provides four orders of magnitude performance improvement. Time-resolved detection allows discrimination of system and target fluorescence.

Conference Presentation

Citation Download Citation

Eric Brace and Pierre Lane "Quantification and improvement of signal-to-background ratio of endogenous fluorescence imaging using double-clad fiber endoscopes", Proc. SPIE PC12356, Endoscopic Microscopy XVIII, PC123560N (17 March 2023); https://doi.org/10.1117/12.2650518

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