Label free multi-photon metabolic imaging: from mitochondria to humans

Irene Georgakoudi; Christopher Polleys; Yang Zhang; Volha Liaudanskaya; Adriana Sanchez; Einstein Gnanatheepam; Maria Savvidou; David Kaplan; Hong-Thao Thieu; Elizabeth Genega; Thomas Schenlldorfer; Jessica Shiu; Anand Ganesan; Mihaela Balu

doi:10.1117/12.2658901

17 March 2023 Label free multi-photon metabolic imaging: from mitochondria to humans

Irene Georgakoudi, Christopher Polleys, Yang Zhang, Volha Liaudanskaya, Adriana Sanchez, Einstein Gnanatheepam, Maria Savvidou, David Kaplan, Hong-Thao Thieu, Elizabeth Genega, Thomas Schenlldorfer, Jessica Shiu, Anand Ganesan, Mihaela Balu

Author Affiliations +

Proceedings Volume PC12363, Multiscale Imaging and Spectroscopy IV; PC123630G (2023) https://doi.org/10.1117/12.2658901
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Multi-photon excited intensity and lifetime fluorescence images relying on endogenous contrast can be analyzed to quantify contributions from key metabolic co-enzymes and associated metabolic function and mitochondrial organization metrics. The high spatio-temporal resolution and context of these non-destructive measurements can be used to provide important insights related to a wide range of samples, conditions and disease models. Corresponding images are acquired from mitochondria, engineered tissues, excised and in vivo human tissues. Recent studies highlight the value of multi-parametric, label-free, metabolic assessments to improve our understanding of traumatic brain injury, (pre)cancer development, and vitiligo lesions.

Conference Presentation

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Irene Georgakoudi, Christopher Polleys, Yang Zhang, Volha Liaudanskaya, Adriana Sanchez, Einstein Gnanatheepam, Maria Savvidou, David Kaplan, Hong-Thao Thieu, Elizabeth Genega, Thomas Schenlldorfer, Jessica Shiu, Anand Ganesan, and Mihaela Balu "Label free multi-photon metabolic imaging: from mitochondria to humans", Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630G (17 March 2023); https://doi.org/10.1117/12.2658901

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