Label-free ex-vivo animal tissue metabolism investigation using dynamic optical coherence tomography

Pradipta Mukherjee; Shinichi Fukuda; Donny Lukmanto; Toshiharu Yamashita; Kosuke Okada; Shuichi Makita; Ibrahim Abd El-Sadek; Arata Miyazawa; Yoshiaki Yasuno

doi:10.1117/12.2649592

27 March 2023 Label-free ex-vivo animal tissue metabolism investigation using dynamic optical coherence tomography

Pradipta Mukherjee, Shinichi Fukuda, Donny Lukmanto, Toshiharu Yamashita, Kosuke Okada, Shuichi Makita, Ibrahim Abd El-Sadek, Arata Miyazawa, Yoshiaki Yasuno

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Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671J (2023) https://doi.org/10.1117/12.2649592
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Tissue metabolism is an inter-and intra-cellular process, which is essential to maintain the living state of tissue. Functional abnormality of such cellular processes initiates several metabolic diseases including non-alcoholic-fatty-liver-disease (NAFLD), liver fibrosis, cancer, chronic kidney disease, and so on. To understand the metabolic processes and how tissue metabolism changes from healthy to diseased conditions, it is required to investigate tissue metabolic activity in animal models in a label-free manner. Since the metabolic structures in animal organs extend into the deep region, deep tissue metabolism imaging is necessary. In this paper, we demonstrate the investigation of ex vivo animal organ models’ metabolism including mouse liver and kidney tissues using OCT-based dynamics imaging method. The dynamics imaging method is based on logarithmic intensity variance (LIV) analysis that enables volumetric metabolic imaging with a 50-kHz swept-source OCT system. NAFLD model mouse livers and obstructed mouse kidney model were investigated ex vivo. In the normal liver, highly dynamic vessel-like structures were observed and they might correspond to high metabolic activity in the periportal/perivenous zones. In the 1-week NAFLD model, a macroscopic, ring-shaped dynamic structure was observed which may indicate highly-motile lipid droplets. In the 2-week NAFLD model, highly-dynamic fragmented vessel-like structures were observed, which are believed to correspond to inflammatory cells around large vessels. In the normal mouse kidney, superficial pipe-like metabolic structures were observed that may correspond to the kidney renal tubules. The pipe-like structures did not appear in the obstructed kidney model.

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Pradipta Mukherjee, Shinichi Fukuda, Donny Lukmanto, Toshiharu Yamashita, Kosuke Okada, Shuichi Makita, Ibrahim Abd El-Sadek, Arata Miyazawa, and Yoshiaki Yasuno "Label-free ex-vivo animal tissue metabolism investigation using dynamic optical coherence tomography", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671J (27 March 2023); https://doi.org/10.1117/12.2649592

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