Validating polarization-sensitive OCT images of the human tympanic membrane by polarized light microscopy (Conference Presentation)

Svea Steuer; Jonas Golde; Lars Kirsten; Joseph Morgenstern; Anett Jannasch; Marcus Neudert; Edmund Koch

doi:10.1117/12.2650255

15 March 2023 Validating polarization-sensitive OCT images of the human tympanic membrane by polarized light microscopy (Conference Presentation)

Svea Steuer, Jonas Golde, Lars Kirsten, Joseph Morgenstern, Anett Jannasch, Marcus Neudert, Edmund Koch

Author Affiliations +

Proceedings Volume PC12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023; PC123820I (2023) https://doi.org/10.1117/12.2650255
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Imaging the collagen layer of the human tympanic membrane, on which the human hearing ability depends, is possible in vivo by using endoscopic polarization-sensitive optical coherence tomography (PSOCT), as we have shown recently. Since the resolution of the utilized system was limited, an ex vivo validation of the structural information is presented here. By comparing higher resolution PSOCT images of a temporal bone specimen and polarized light microscopy (PLM) of histological slices cut from the same sample and stained with picrosirius red, the microstructure is investigated. Besides matching PSOCT and PLM images, the methodical differences are discussed.

Conference Presentation

Citation Download Citation

Svea Steuer, Jonas Golde, Lars Kirsten, Joseph Morgenstern, Anett Jannasch, Marcus Neudert, and Edmund Koch "Validating polarization-sensitive OCT images of the human tympanic membrane by polarized light microscopy (Conference Presentation)", Proc. SPIE PC12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023, PC123820I (15 March 2023); https://doi.org/10.1117/12.2650255

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