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15 March 2023 Measuring mass and loading of single carboxysomes in the interferometric scattering anti-Brownian electrokinetic trap (Conference Presentation)
Abhijit A. Lavania, William B. Carpenter, Luke M. Oltrogge, Davis D. Perez, Julia Borden, David F. Savage, William E. Moerner
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Proceedings Volume PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; PC1238601 (2023) https://doi.org/10.1117/12.2650912
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Anti-Brownian traps confine single molecules or particles in free solution by closed-loop feedback forces, allowing detailed characterization of photophysical and transport properties. We have recently extended this approach to tracking the particles with interferometric scattering at near infrared wavelengths. This extension allows trapping non-fluorescent particles and performing simultaneous modulated fluorescence measurements in the Interferometric Scattering Anti-Brownian ELectrokinetic (ISABEL) trap. Here we use the interferometric scattering signal in the ISABEL trap to measure the scattering cross-sections of single carboxysomes, bacterial nanocompartments involved in carbon fixation. With a core-shell model, we can calculate the total mass and internal loading of single nano-objects.
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Abhijit A. Lavania, William B. Carpenter, Luke M. Oltrogge, Davis D. Perez, Julia Borden, David F. Savage, and William E. Moerner "Measuring mass and loading of single carboxysomes in the interferometric scattering anti-Brownian electrokinetic trap (Conference Presentation)", Proc. SPIE PC12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, PC1238601 (15 March 2023); https://doi.org/10.1117/12.2650912
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KEYWORDS
Scattering
Interferometry
Luminescence
Light scattering
Particles
Proteins
Infrared detectors
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