Comparison of the effectiveness of two different light spectra in 5-ALA-based antimicrobial photodynamic therapy of orthopaedic implant-relevant biofilms

Logan M. Bateman; Valentin V. Demidov; Natalia Demidova; Jason R. Gunn; Scott J. Sottosanti; Eric R. Henderson; Ida L. Gitajn; Jonathan T. Elliot

doi:10.1117/12.3003128

13 March 2024 Comparison of the effectiveness of two different light spectra in 5-ALA-based antimicrobial photodynamic therapy of orthopaedic implant-relevant biofilms

Logan M. Bateman, Valentin V. Demidov, Natalia Demidova, Jason R. Gunn, Scott J. Sottosanti, Eric R. Henderson, Ida L. Gitajn, Jonathan T. Elliot

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Proceedings Volume PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; PC128220G (2024) https://doi.org/10.1117/12.3003128
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Bacterial biofilms are a pervasive issue in orthopaedic surgery causing up to 80% of infections. Antimicrobial photodynamic therapy (aPDT) is a proposed technique for eradication of biofilms, clinical translation requires optimization of treatment parameters. This study assessed the effectiveness of three light spectra in activating photosensitizing porphyrins to kill a dual species biofilm of E. Coli and E. faecalis grown in a microfluidic device. Blue-red, amber, or blue-amber-red light sources were compared at either 30J/cm2 or 60J/cm2 doses given to activate endogenously produced porphyrins after one hour incubation with 10 or 20% 5-ALA in a saline solution. Changes in biomass 24 hours after treatment were measured using confocal microscopy and OCT to determine treatment effectiveness.

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Logan M. Bateman, Valentin V. Demidov, Natalia Demidova, Jason R. Gunn, Scott J. Sottosanti, Eric R. Henderson, Ida L. Gitajn, and Jonathan T. Elliot "Comparison of the effectiveness of two different light spectra in 5-ALA-based antimicrobial photodynamic therapy of orthopaedic implant-relevant biofilms", Proc. SPIE PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, PC128220G (13 March 2024); https://doi.org/10.1117/12.3003128

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KEYWORDS

Biofilms

Photodynamic therapy

Biomass

Light sources

Optical coherence tomography

Oxygen

Surgery

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