Spatially-resolved phase signal extraction in OCT reveals distinct light-evoked responses of photoreceptors and retinal pigment epithelium

Tong Ling; Bingyao Tan; Huakun Li; Yueming Zhuo; Le Han; Rajeshkumar Mupparapu; Veluchamy Amutha Barathi; Daniel Palanker; Leopold Schmetterer

doi:10.1117/12.3005552

13 March 2024 Spatially-resolved phase signal extraction in OCT reveals distinct light-evoked responses of photoreceptors and retinal pigment epithelium

Tong Ling, Bingyao Tan, Huakun Li, Yueming Zhuo, Le Han, Rajeshkumar Mupparapu, Veluchamy Amutha Barathi, Daniel Palanker, Leopold Schmetterer

Author Affiliations +

Proceedings Volume PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; PC1283002 (2024) https://doi.org/10.1117/12.3005552
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Previous studies in optoretinography often rely on AO-OCT systems to resolve individual cells or use intensity-based image processing to extract the dynamics of the retinal layer as an ensemble. For non-AO point-scan OCT, investigating tissue dynamics from obscure speckle patterns while preserving the spatial heterogeneity of signals remains challenging. Here, we developed phase-restoring subpixel image registration and unsupervised machine learning algorithms to accurately extract spatially-resolved OCT phase signals from the outer retina in rodents. In addition to observing light-evoked deformation of the photoreceptors outer segments, we discovered an optical signature of the retinal pigment epithelium (RPE) response to visual stimuli.

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Tong Ling, Bingyao Tan, Huakun Li, Yueming Zhuo, Le Han, Rajeshkumar Mupparapu, Veluchamy Amutha Barathi, Daniel Palanker, and Leopold Schmetterer "Spatially-resolved phase signal extraction in OCT reveals distinct light-evoked responses of photoreceptors and retinal pigment epithelium", Proc. SPIE PC12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, PC1283002 (13 March 2024); https://doi.org/10.1117/12.3005552

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