Fluorescence lifetime imaging (FLIM) is a popular and versatile tool for in vivo research with minimal disruption of the biological system. Recently, the desire has grown to investigate dynamic processes with FLIM, creating a need for video rate FLIM contrast measurements. Because the potential applications are manifold, the different information depth of video FLIM and ‘detailed, slow’ FLIM measurements must be considered.
This talk highlights the complimentary nature of video rate and standard FLIM measurements with practical examples, measured with the same machine. Strengths and limitations of each measurement mode are highlighted, and best-practice analysis strategies are determined for each.
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