Fast volumetric imaging in tissue

Jerome Mertz

doi:10.1117/12.3016422

13 March 2024 Fast volumetric imaging in tissue

Jerome Mertz

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC1284805 (2024) https://doi.org/10.1117/12.3016422
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Obtaining high resolution images in scattering tissue has been a long-standing challenge in microscopy. This challenge is exacerbated when high frame rates are required, or when 3D imaging is required over extended volumes. I will present several tools we have developed to address this challenge. These can be applied to a variety of microscopy modalities ranging from multiplane imaging with speckle, to multi-focus confocal imaging with targeted illumination, and finally to multiphoton imaging. Our main applications are in-vivo imaging in the mouse brain, where we demonstrate high contrast kHz-rate imaging of neuronal electrical activity and blood flow deep in tissue.

Conference Presentation

Citation Download Citation

Jerome Mertz "Fast volumetric imaging in tissue", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC1284805 (13 March 2024); https://doi.org/10.1117/12.3016422

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