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13 March 2024 Video-rate mid-infrared photothermal imaging by single-pulse photothermal detection per pixel
Jiaze Yin, Meng Zhang, Yuying Tan, Zhongyue Guo, Hongjian He, Lu Lan, Ji-Xin Cheng
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Proceedings Volume PC12853, High-Speed Biomedical Imaging and Spectroscopy IX; PC128530H (2024) https://doi.org/10.1117/12.3003528
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
The imaging speed of current mid-infrared photothermal (MIP) microscope is limited to tens of seconds per frame due to the long pixel dwell time and slow sample scanning process, which is insufficient for capturing dynamics inside living systems. In this work, we developed a video-rate MIP microscope by employing a lock-in free demodulation scheme to resolve single IR pulse induced contrast. We further developed a synchronous pump-probe Galvo scanning for reaching a line rate over 2kHz. With such scheme, the system is capable of resolving chemical dynamics of various biomolecules in living organisms at multiple scales.
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Jiaze Yin, Meng Zhang, Yuying Tan, Zhongyue Guo, Hongjian He, Lu Lan, and Ji-Xin Cheng "Video-rate mid-infrared photothermal imaging by single-pulse photothermal detection per pixel", Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530H (13 March 2024); https://doi.org/10.1117/12.3003528
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KEYWORDS
Mid-IR
Biological imaging
Infrared imaging
Microscopes
Photothermal effect
Pulse signals
Pulsed laser operation
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