Phase locking of temporally filtered SRS signals for label-free small-molecule detection at sub-millimolar concentrations

Terumasa Ito; Fumiaki Matsuoka; Kazuhiko Misawa

doi:10.1117/12.3002835

13 March 2024 Phase locking of temporally filtered SRS signals for label-free small-molecule detection at sub-millimolar concentrations

Terumasa Ito, Fumiaki Matsuoka, Kazuhiko Misawa

Author Affiliations +

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC1285509 (2024) https://doi.org/10.1117/12.3002835
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) microscopy offers label-free, high-speed measurements of small molecules in living cells and tissues, holding promise for potential biomedical applications such as drug screening and therapeutic drug monitoring. Nevertheless, with conventional SRS microscopy, fluctuations in the background Raman signal from cells and tissues limit quantitative measurements at drug concentrations below a few mM. Here we present novel SRS microscopy using temporal filtering combined with a phase-locking technique to break the detection limit due to the background Raman signals. We demonstrate that the new phase-locked system enables drug measurements at sub millimolar concentrations with linear calibration curves.

Conference Presentation

Citation Download Citation

Terumasa Ito, Fumiaki Matsuoka, and Kazuhiko Misawa "Phase locking of temporally filtered SRS signals for label-free small-molecule detection at sub-millimolar concentrations", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC1285509 (13 March 2024); https://doi.org/10.1117/12.3002835

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Electronic filtering

Tunable filters

Signal detection

Mode locking

Raman spectroscopy

Microscopy

Frequency modulation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years