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13 March 2024 Rapid identification and antimicrobial susceptibility testing in urinary tract infection by FISH-SRS
Meng Zhang, Hongli Ni, Xiaowei Ge, Lu Lan, Mohamed Seleem, Michael Wagner, Ji-Xin Cheng
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550F (2024) https://doi.org/10.1117/12.3003454
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Timely and accurate identification and antimicrobial susceptibility testing (AST) is essential to slow down the emergence of antimicrobial resistance and consequently reduce deaths due to drug-resistant infections. Here, we present multi-color fluorescence in situ hybridization (FISH) and stimulated Raman scattering (SRS) metabolic imaging to enable rapid identification and AST (iAST) of clinical samples. We have successfully developed FISH probes that utilizes oligonucleotide probes targeting the ribosomal RNA (rRNA) of bacteria strains associated with urinary tract infections (UTIs), which enables rapid and direct single-cell identification of microbes. By fast tuning between C-D and C-H vibration, we are able to use CD/(CD+CH) SRS signal ratio to quantitate the intracellular deuterium oxide (D2O) metabolism at single cell level. High-throughput, high-speed AST through robotic handling of liquid specimens and a multi-well chamber design has been demonstrated. We test the efficacy of our approach on common UTI associated bacterial isolates and clinical specimens, and demonstrate a 2 h diagnostic time, with an identification and categorical susceptible/resistant accuracy over 95%.
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Meng Zhang, Hongli Ni, Xiaowei Ge, Lu Lan, Mohamed Seleem, Michael Wagner, and Ji-Xin Cheng "Rapid identification and antimicrobial susceptibility testing in urinary tract infection by FISH-SRS", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550F (13 March 2024); https://doi.org/10.1117/12.3003454
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KEYWORDS
Liquids
Microorganisms
Microscopes
Mode conditioning cables
Oxides
Raman scattering
Resistance
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