Multi-dimensional widefield infrared-encoded spontaneous emission (MD-WISE) microscopy

Wei Xiong; Chang Yan; Chenglai Wang; Jianyu Ren

doi:10.1117/12.3003566

13 March 2024 Multi-dimensional widefield infrared-encoded spontaneous emission (MD-WISE) microscopy

Wei Xiong, Chang Yan, Chenglai Wang, Jianyu Ren

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550U (2024) https://doi.org/10.1117/12.3003566
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Introducing MD-WISE microscopy, a groundbreaking technique that extends the applications of Photoluminescence (PL) imaging. While PL imaging is widely used for visualizing biological activities, detecting chemical species, and characterizing materials, its effectiveness is limited due to overlapping emission spectra of chromophores. However, MD-WISE overcomes this challenge by utilizing nonlinear interactions between mid-infrared and visible excitations, enabling the differentiation of chromophores with similar spectra in a multi-dimensional space. This is achieved through two mechanisms: modulating the optical absorption cross sections of molecular dyes by exciting specific vibrational groups and reducing the Photoluminescence quantum yield of semiconductor nanocrystals. Unlike most nonlinear optical microscopies that require tightly focused beams, MD-WISE operates under widefield imaging conditions, offering a field of view of 30 by 30 microns. By capturing multi-dimensional information in PL images, MD-WISE microscopy has the potential to revolutionize high-speed widefield imaging by simultaneously tracking numerous species and processes.

Conference Presentation

Citation Download Citation

Wei Xiong, Chang Yan, Chenglai Wang, and Jianyu Ren "Multi-dimensional widefield infrared-encoded spontaneous emission (MD-WISE) microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550U (13 March 2024); https://doi.org/10.1117/12.3003566

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