1 March 2010 Intramyocardial oxygen transport by quantitative diffuse reflectance spectroscopy in calves
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J. of Biomedical Optics, 15(2), 027009 (2010). doi:10.1117/1.3374050
Abstract
Intramyocardial oxygen transport was assessed during open-chest surgery in calves by diffuse reflectance spectroscopy using a small intramuscular fiber-optic probe. The sum of hemo- and myoglobin tissue fraction and oxygen saturation, the tissue fraction and oxidation of cytochrome aa3, and the tissue fraction of methemoglobin were estimated using a calibrated empirical light transport model. Increasing the oxygen content in the inhaled gas, 21%-50%-100%, in five calves (group A) gave an increasing oxygen saturation of 19±4%, 24±5%, and 28±8% (p<0.001, ANOVA repeated measures design) and mean tissue fractions of 1.6% (cytochrome aa3) and 1.1% (hemo- and myoglobin). Cardiac arrest in two calves gave an oxygen saturation lower than 5%. In two calves (group B), a left ventricular assistive device (LVAD pump) was implanted. Oxygen saturation in group B animals increased with LVAD pump speed (p<0.001, ANOVA) and with oxygen content in inhaled gas (p<0.001, ANOVA). The cytochrome aa3 oxidation level was above 96% in both group A and group B calves, including the two cases involving cardiac arrest. In conclusion, the estimated tissue fractions and oxygenation/oxidation levels of the myocardial chromophores during respiratory and hemodynamic provocations were in agreement with previously presented results, demonstrating the potential of the method.
Tobias Lindbergh, Marcus Larsson, Zoltán Szabó, Henrik Casimir-Ahn, Tomas Strömberg, "Intramyocardial oxygen transport by quantitative diffuse reflectance spectroscopy in calves," Journal of Biomedical Optics 15(2), 027009 (1 March 2010). http://dx.doi.org/10.1117/1.3374050
Submission: Received ; Accepted
JOURNAL ARTICLE
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KEYWORDS
Oxygen

Tissues

Heart

Chromophores

Absorption

Diffuse reflectance spectroscopy

Oxidation

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