Wavelength selection in measuring red blood cell aggregation based on light transmittance

Mehmet Uyuklu; Murat Canpolat; Herbert J. Meiselman; Oguz K. Baskurt

doi:10.1117/1.3652712

1 November 2011 Wavelength selection in measuring red blood cell aggregation based on light transmittance

Mehmet Uyuklu, Murat Canpolat, Herbert J. Meiselman, Oguz K. Baskurt

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Journal of Biomedical Optics, Vol. 16, Issue 11, 117006 (November 2011). https://doi.org/10.1117/1.3652712

Abstract

The reversible aggregation of red blood cells (RBC) is of current basic science and clinical interest. Using a flow channel and light transmittance (LT) through RBC suspensions, we have examined the effects of wavelength (500 to 900 nm) on the static and dynamic aspects of RBC aggregation for normal blood and suspensions with reduced or enhanced aggregation; the effects of oxygenation were also explored. Salient observations include: 1. significant effects of wavelength on aggregation parameters reflecting the extent of aggregation (i.e., number of RBC per aggregate); 2. no significant effects of wavelength on parameters reflecting the time course of RBC aggregation; 3. a prominent influence of hemoglobin oxygen saturation on both extent and time-course related aggregation parameters measured at wavelengths less than 700 nm, but only on the time-course at 800 nm; and 4. the power of parameters in detecting a given alteration of RBC aggregation is affected by wavelength, in general being greater at higher wavelengths. It is recommended that light sources with wavelengths around 800 nm be used in instruments for measuring RBC aggregation via LT.

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Mehmet Uyuklu, Murat Canpolat, Herbert J. Meiselman, and Oguz K. Baskurt "Wavelength selection in measuring red blood cell aggregation based on light transmittance," Journal of Biomedical Optics 16(11), 117006 (1 November 2011). https://doi.org/10.1117/1.3652712

Published: 1 November 2011

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