Lensless fluorescence imaging with height calculation

Akshaya Shanmugam; Christopher D. Salthouse

doi:10.1117/1.JBO.19.1.016002

3 January 2014 Lensless fluorescence imaging with height calculation

Akshaya Shanmugam, Christopher D. Salthouse

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Journal of Biomedical Optics, Vol. 19, Issue 1, 016002 (January 2014). https://doi.org/10.1117/1.JBO.19.1.016002

Abstract

Lensless fluorescence imaging (LFI) is the imaging of fluorescence from cells or microspheres using an image sensor with no external lenses or filters. The simplicity of the hardware makes it well suited to replace fluorescence microscopes and flow cytometers in lab-on-a-chip applications, but the images captured by LFI are highly dependent on the distance between the sample and the sensor. This work demonstrates that not only can samples be accurately detected across a range of sample-sensor separations using LFI, but also that the separation can be accurately estimated based on the shape of fluorescence in the LFI image. First, a theoretical model that accurately predicts LFI images of microspheres is presented. Then, the experimental results are compared to the model and an image processing method for accurately predicting sample-sensor separation from LFI images is presented. Finally, LFI images of microspheres and cells passing through a microfluidic channel are presented.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Akshaya Shanmugam and Christopher D. Salthouse "Lensless fluorescence imaging with height calculation," Journal of Biomedical Optics 19(1), 016002 (3 January 2014). https://doi.org/10.1117/1.JBO.19.1.016002

Published: 3 January 2014

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