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15 December 2014 Extension of the concept of an anomalous water component to images of T-cell organelles
Vladimir P. Tychinsky
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Journal of Biomedical Optics, Vol. 19, Issue 12, 126008 (December 2014). https://doi.org/10.1117/1.JBO.19.12.126008
Abstract
Microscopic images of a living cell are the main source of information on its functional state. Modern interference microscopy techniques allow the numerical parameters of cell images to be obtained with an accuracy not available with other methods. Quantitative analysis of phase images of T lymphocytes (TCs) in different functional states demonstrated that variations of the properties of intracellular water should be taken into account. This conclusion agrees with the current view that the physical parameters of water, including the refractive index (RI) of a water layer, depend on the hydrophilicity and other characteristics of the adjacent surface. Application of this concept to phase images of TCs showed that the contribution of the fourth phase of water (4-water) or the structured water component, which has an increased RI, should be considered. The proportion of 4-water depends on the functional state of the cell determined by the culture medium composition. Normally, the proportion of 4-water in organelles is as high as 30%; it is considerably lower in organelles of cells with inhibited metabolism.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Vladimir P. Tychinsky "Extension of the concept of an anomalous water component to images of T-cell organelles," Journal of Biomedical Optics 19(12), 126008 (15 December 2014). https://doi.org/10.1117/1.JBO.19.12.126008
Published: 15 December 2014
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KEYWORDS
Water
Proteins
Neodymium
Technetium
Microscopes
Refractive index
Microscopy
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