Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture

Rebekah A. Drezek; Martial Guillaud; Thomas G. Collier; Iouri Boiko; Anais Malpica M.D.; Calum E. MacAulay; Michele Follen; Rebecca R. Richards-Kortum

doi:10.1117/1.1528950

1 January 2003 Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture

Rebekah A. Drezek, Martial Guillaud, Thomas G. Collier, Iouri Boiko, Anais Malpica M.D., Calum E. MacAulay, Michele Follen, Rebecca R. Richards-Kortum

Author Affiliations +

Journal of Biomedical Optics, Vol. 8, Issue 1, (January 2003). https://doi.org/10.1117/1.1528950

A number of noninvasive fiber optic optical technologies are under development for real-time diagnosis of neoplasia. We investigate how the light scattering properties of cervical cells are affected by changes in nuclear morphology, DNA content, and chromatin texture, which occur during neoplastic progression. We used a Cyto-Savant computer-assisted image analysis system to acquire quantitative nuclear features measurements from 122 Feulgen-thionin-stained histopathologic sections of cervical tissue. A subset of the measured nuclear features was incorporated into a finite-difference time-domain (FDTD) model of cellular light scattering. The magnitude and angular distribution of scattered light was calculated for cervical cells as a function of pathologic grade. The nuclear atypia strongly affected light scattering properties. The increased size and elevated DNA content of nuclei in high-grade lesions caused the most significant changes in scattering intensity. The spatial dimensions of chromatin texture features and the amplitude of refractive index fluctuations within the nucleus impacted both the angular distribution of scattering angles and the total amount of scattered light. Cellular scattering is sensitive to changes in nuclear morphology that accompany neoplastic progression. Understanding the quantitative relationships between nuclear features and scattering properties will aid in the development of noninvasive optical technologies for detection of precancerous conditions.

©(2003) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Rebekah A. Drezek, Martial Guillaud, Thomas G. Collier, Iouri Boiko, Anais Malpica M.D., Calum E. MacAulay, Michele Follen, and Rebecca R. Richards-Kortum "Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture," Journal of Biomedical Optics 8(1), (1 January 2003). https://doi.org/10.1117/1.1528950

Published: 1 January 2003

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY