Aggregation of nanoparticles in endosomes and lysosomes produces surface-enhanced Raman spectroscopy

Leanne Lucas; Xiaoke K. Chen; Aaron J. Smith; Mladen Korbelik; Haishan Zeng; Patrick W. K. Lee; Kevin C. Hewitt

doi:10.1117/1.JNP.9.093094

23 January 2015 Aggregation of nanoparticles in endosomes and lysosomes produces surface-enhanced Raman spectroscopy

Leanne Lucas, Xiaoke K. Chen, Aaron J. Smith, Mladen Korbelik, Haishan Zeng, Patrick W. K. Lee, Kevin C. Hewitt

Author Affiliations +

Journal of Nanophotonics, Vol. 9, Issue 1, 093094 (January 2015). https://doi.org/10.1117/1.JNP.9.093094

Abstract

The purpose of this study was to explore the use of surface-enhanced Raman spectroscopy (SERS) to image the distribution of epidermal growth factor receptor (EGFR) in cells. To accomplish this task, 30-nm gold nanoparticles (AuNPs) tagged with antibodies to EGFR (10¹² per mL) were incubated with cells (10⁶ per mL) of the A431 human epidermoid carcinoma and normal human bronchial epithelial cell lines. Using the 632.8-nm excitation line of a He-Ne laser, Raman spectroscopy measurements were performed using a point mapping scheme. Normal cells show little to no enhancement. SERS signals were observed inside the cytoplasm of A431 cells with an overall enhancement of 4 to 7 orders of magnitude. Raman intensity maps of the 1450 and 1583 cm−¹ peaks correlate well with the expected distribution of EGFR and AuNPs, aggregated following uptake by endosomes and lysosomes. Spectral features from tyrosine and tryptophan residues dominate the SERS signals.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Citation Download Citation

Leanne Lucas, Xiaoke K. Chen, Aaron J. Smith, Mladen Korbelik, Haishan Zeng, Patrick W. K. Lee, and Kevin C. Hewitt "Aggregation of nanoparticles in endosomes and lysosomes produces surface-enhanced Raman spectroscopy," Journal of Nanophotonics 9(1), 093094 (23 January 2015). https://doi.org/10.1117/1.JNP.9.093094

Published: 23 January 2015

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
14 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY