Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons

Alec M. Degrand; Stephen J. Lomnes; Deborah S. Lee; Matthew Pietrzykowski; Shunsuke Ohnishi; Timothy Morgan; Andrew Gogbashian; Rita G. Laurence; John V. Frangioni M.D.

doi:10.1117/1.2170579

1 January 2006 Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons

Alec M. Degrand, Stephen J. Lomnes, Deborah S. Lee, Matthew Pietrzykowski, Shunsuke Ohnishi, Timothy Morgan, Andrew Gogbashian, Rita G. Laurence, John V. Frangioni M.D.

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Journal of Biomedical Optics, Vol. 11, Issue 1, 014007 (January 2006). https://doi.org/10.1117/1.2170579

Abstract

We demonstrate how to construct calibrated, stable, and inexpensive tissue-like phantoms for near-IR (NIR) fluorescence imaging applications. The bulk phantom material is composed of gelatin, intralipid, hemoglobin, and indocyanine green (ICG). Absorbance, scatter, background fluorescence, and texture can be tuned as desired. NIR fluorescent inclusions are comprised of ICG-labeled polystyrene divinylbenzene beads and Pam78-labeled hydroxyapatite crystals. The former mimic tumor masses of controllable size and contrast agent concentration, and the latter mimic microcalcifications in breast cancer. NIR-fluorescent inclusions can be positioned precisely in phantoms, with one or more regions having different optical properties, and their position can be verified independently using microcomputed tomography. We demonstrate how these phantoms can be used to calibrate and compare imaging systems, and to train surgeons to operate under NIR fluorescence image guidance.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Alec M. Degrand, Stephen J. Lomnes, Deborah S. Lee, Matthew Pietrzykowski, Shunsuke Ohnishi, Timothy Morgan, Andrew Gogbashian, Rita G. Laurence, and John V. Frangioni M.D. "Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons," Journal of Biomedical Optics 11(1), 014007 (1 January 2006). https://doi.org/10.1117/1.2170579

Published: 1 January 2006

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