Quantum dot optical encoded polystyrene beads for DNA detection

Yuan-Cheng Cao; Tian-Cai Liu; Xiao-Feng Hua; Xiao-Xia Zhu; Hai-Qiao Wang; Zhen-Li Huang; Yuan-Di Zhao; Man-Xi Liu; Qingming Luo

doi:10.1117/1.2358957

1 September 2006 Quantum dot optical encoded polystyrene beads for DNA detection

Yuan-Cheng Cao, Tian-Cai Liu, Xiao-Feng Hua, Xiao-Xia Zhu, Hai-Qiao Wang, Zhen-Li Huang, Yuan-Di Zhao, Man-Xi Liu, Qingming Luo

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Journal of Biomedical Optics, Vol. 11, Issue 5, 054025 (September 2006). https://doi.org/10.1117/1.2358957

Abstract

A novel multiplex analysis technology based on quantum dot (QD) optical encoded beads was studied. Carboxyl functionalized polystyrene beads, about 100 µm in size, were precisely encoded by the various ratios of two types of QDs whose emission wavelengths are 576 and 628 nm, respectively. Then the different encoded beads were covalently immobilized with different probes in the existing of sulfo-NHS and 1-[3-(Dimethylamino) propyl]-3-ethylcarbodiimide methiodide, and the probe density could reach to 3.1 mmol/g. These probe-linked encoded beads were used to detect the target DNA sequences in complex DNA solution by hybridization. Hybridization was visualized using fluorescein isothiocynate–labeled DNA sequences. The results show that the QDs and target signals can be obviously identified from a single-bead-level spectrum. This technology can detect DNA targets effectively with a detection limit of 0.2 µg/mL in complex solution.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Yuan-Cheng Cao, Tian-Cai Liu, Xiao-Feng Hua, Xiao-Xia Zhu, Hai-Qiao Wang, Zhen-Li Huang, Yuan-Di Zhao, Man-Xi Liu, and Qingming Luo "Quantum dot optical encoded polystyrene beads for DNA detection," Journal of Biomedical Optics 11(5), 054025 (1 September 2006). https://doi.org/10.1117/1.2358957

Published: 1 September 2006

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