Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection

Carsten Glasenapp; Wolfgang Monch; Holger Krause; Hans Zappe

doi:10.1117/1.2437143

1 January 2007 Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection

Carsten Glasenapp, Wolfgang Monch, Holger Krause, Hans Zappe

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Journal of Biomedical Optics, Vol. 12, Issue 1, 014038 (January 2007). https://doi.org/10.1117/1.2437143

Abstract

A highly parallel microarray scanner for functional genomic research using a dynamically reconfigurable holographic excitation and hyperspectral fluorescence detection is described. The light from two laser sources (405 and 532 nm) is split into an arbitrary number of focused spots on the biochip using a pair of spatial light modulators and a novel imaging system. The parallel optical scanner includes a hyperspectral detection unit with a high-sensitivity CCD camera to detect and analyze the emitted fluorescence spectra (from 430 to 800 nm) of all illuminated spots simultaneously. By using an xy scanner, the spectra of all spots on an entire array can be read out line by line.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Carsten Glasenapp, Wolfgang Monch, Holger Krause, and Hans Zappe "Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection," Journal of Biomedical Optics 12(1), 014038 (1 January 2007). https://doi.org/10.1117/1.2437143

Published: 1 January 2007

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