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1 July 2008 Intermyofilament dynamics of myocytes revealed by second harmonic generation microscopy
Nicole Prent, Chantal Green, Catherine Greenhalgh, Richard Cisek, Arkady Major, Bryan Stewart, Virginijus Barzda
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Journal of Biomedical Optics, Vol. 13, Issue 4, 041318 (July 2008). https://doi.org/10.1117/1.2950316
Abstract
Drosophila melanogaster larva myocytes are imaged with second harmonic generation (SHG) microscopy undergoing forced stretching and rhythmic contractions to determine the nature of the SHG signal. During stretching, double peaked SHG profiles of the anisotropic (A-) bands evolve into single peaks with a higher SHG intensity. The dip in the intensity profile at the center of the A-band is attributed to destructive interference from out-of-phase second harmonic radiating myosin molecules that, in the central region of myofilaments, are arranged antiparallel. An intensity increase at the center of the A-band appears during forced stretching due to a small, less than 100 nm, intermyofilament separation of the antiparallel myosin molecules leading to constructive interference of the SHG radiation. In addition, the same phenomenon occurs during periodic contractions of the myocyte, where an SHG intensity increase with the lengthening of sarcomeres is observed. The SHG intensity dependence on sarcomere length can be used for imaging myocyte contractions with low resolution microscopy, and can be applied for the development of diagnostic tools where monitoring of muscle contraction dynamics is required.
©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Nicole Prent, Chantal Green, Catherine Greenhalgh, Richard Cisek, Arkady Major, Bryan Stewart, and Virginijus Barzda "Intermyofilament dynamics of myocytes revealed by second harmonic generation microscopy," Journal of Biomedical Optics 13(4), 041318 (1 July 2008). https://doi.org/10.1117/1.2950316
Published: 1 July 2008
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KEYWORDS
Second-harmonic generation
Molecules
Microscopy
Harmonic generation
Microscopes
Constructive interference
Diagnostics
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