Ultrasensitive protein detection in blood serum using gold nanoparticle probes by single molecule spectroscopy

Jiji Chen; Chungang Wang; Joseph M. Irudayaraj

doi:10.1117/1.3183789

1 July 2009 Ultrasensitive protein detection in blood serum using gold nanoparticle probes by single molecule spectroscopy

Jiji Chen, Chungang Wang, Joseph M. Irudayaraj

Author Affiliations +

Journal of Biomedical Optics, Vol. 14, Issue 4, 040501 (July 2009). https://doi.org/10.1117/1.3183789

Abstract

A one-step rapid and ultrasensitive immunoassay capable of detecting proteins in blood serum is developed using gold nanoprobes and fluorescence correlation spectroscopy (FCS). In this approach we take advantage of the inherent photoluminescence property of gold nanoparticles (GNPs) to develop a fluorophore-free assay to observe binding entities by monitoring the diffusion of bound versus unbound molecules in a limited confocal volume. 40-nm GNPs conjugated separately with rabbit anti-IgG (Fc) and goat anti-IgG (Fab) when incubated in blood serum containing IgG forms a sandwich structure constituting dimers and oligomers that can be differentiated by to detect IgG in blood serum at a limit of detection (LOD) of 5 pg/ml. The novelty of integrating GNPs with FCS to develop a sensitive blood immunoassay brings single molecule methods one step closer to the clinic.

©(2009) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Jiji Chen, Chungang Wang, and Joseph M. Irudayaraj "Ultrasensitive protein detection in blood serum using gold nanoparticle probes by single molecule spectroscopy," Journal of Biomedical Optics 14(4), 040501 (1 July 2009). https://doi.org/10.1117/1.3183789

Published: 1 July 2009

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Cited by 22 scholarly publications and 1 patent.

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KEYWORDS

Diffusion

Blood

Fluorescence correlation spectroscopy

Gold

Nanoparticles

Proteins

Molecules

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