Early stage pressure ulcers and other subcutaneous lesions are nearly invisible in clinical settings, particularly so for dark pigmented skin. An earlier study indicated multispectral images can enhance the contrast between damaged and normal skin. Although multispectral imaging has matured into a technology with applications in many fields, clinical practitioners in the fields of health care and rehabilitation have generally stayed away from it due to extremely high cost and lack of portability. Also, other multispectral imaging technologies require either multiple exposures or extensive postprocessing.
In has long been desirable to reduce the size and weight of multispectral imaging sensors to facilitate their practical applications.1 The effort of developing a hand-held, low-cost, stand-alone, multispectral imaging tool to satisfy the need of clinically detecting early stage pressure ulcers stimulates a new way in architecting the device.2 Instead of using bulky and expensive traditional optical filters, e.g., filter wheels, a generalized Lyot filter, and electrically tunable filter, multiple-band pass filters,3 or the methods of dispersing light, e.g., optic-acoustic crystals,4 we report a custom-designed filter mosaic that has been successfully fabricated using lithography and vacuum multilayer film technologies.
Sprigle, Zhang, and Duckworth5 suggest that three or four wavelengths are sufficient for the detection of early stage pressure ulcers. The filter mosaic allows light of specific wavelengths centered at , , , and to pass through; so, the resulting imager can be best tuned to the characteristic chromospheres existing in pressure ulcers. 540 and correspond to the hemoglobin absorption peaks, gives us a melanin or background image, and 970 targets the water peak plus it gives deep penetration. A bandwidth of is used for the detection of pressure ulcers, although the manufacturing processes can achieve a narrower bandwidth of if needed.
The filter mosaic can be deposited on regular optical glass as a substrate or directly on a complementary metal oxide semiconductor (CMOS) or charge-coupled device (CCD) imaging sensor. Figure 1 shows the first prototype version of the filter. Subsequent improvements to the fabrication process have reduced the spacing (gap and border) between individual filter cells to . The area of a single wavelength cell is and represents a compromise between spatial resolution and initial fabricating cost, although a better spatial resolution of can be achieved. For pressure ulcer detection, this spatial resolution is acceptable, as the target is often very close to the imaging sensor, often within in distance. Figure 1 shows the measured response of the filter mosaic to light.
Integration procedures have been established to laminate the filter of a certain cell size with commercial CCD and CMOS imaging sensors of different pixel sizes. Figure 2 shows a multispectral imager obtained by integrating the prototyped filter mosaic with a CMOS imager MT9M001C12STM with a pixel size produced by Aptina Imaging (Corvalis, Oregon). A United States currency quarter is placed beside the imager to indicate its compact size. The filter mosaic is also integrated with CCD imaging sensors (e.g., ICx205AL, pixel size , produced by Sony, result not shown). For better control of the illumination that targets pressure ulcers, the multispectral imager is further integrated with three LED light sources: one warm light, one green light, and one infrared light [see Fig. 2]. Figure 2 provides one raw image obtained with the resulting compact imaging sensor of a flat sheet illuminated by green light centered at .
Figure 2 gives an enlarged version of a few mosaic filter cells, from which one can see that, as expected, only cells of size that correspond to the spectral component of wavelength are lit up, leaving cells of other spectral components ( , , and ) at rather low intensity. This indicates spatial resolution is inversely related to the number of spectral components, i.e., reduced spectral resolution. Miao and Qi6 provide an interpolation technique to estimate the response to one spectral component (e.g., ) at locations that are occupied by other spectral components (e.g., ) to recover the lost spatial resolution.
The prototyped multispectral imager detects early stage pressure ulcers. An erythema is induced by a pressure ball on dark pigmented skin where it is barely visible to the naked eye (not shown). With a single shot, the integrated multispectral imager produces in real time four images corresponding to wavelengths 540, 577, 650, and , where the erythema becomes visible, although not very clearly [Fig. 3 ]. The real time-fused image is shown in Fig. 3, where the erythema is more apparent than in the single multispectral image. The simple fusion algorithm used to enhance the contrast between erythema and its background is:are because the peak absorption of known characteristic chromophores of pressure ulcers including oxy-and deoxyhemoglobin are at 540 and . The channel is subtracted as it corresponds to melanin content that is constant across erythematic and nonerythematic skin. The spectral component at is not used, as the original imaging sensor MT9M001C12STM has rather low sensitivity in this region. Image processing work with other fusion algorithms and for curvature correction is ongoing.
The reported custom-designed filter mosaic is successfully fabricated and integrated with commercial imaging sensors. The resulting multispectral imager permits multiple images centered at different wavelengths to be acquired in a single exposure, thereby providing overwhelming operational convenience. The real-time production of multispectral images allows instant detection of pressure ulcers. Its capability for real-time detection, miniature size, fully electronic construction with no mechanical components, and immunity from motion-induced distortion facilitates its clinical application in healthcare and rehabilitation fields.
The primary benefits of the core technology reported here, i.e., the new filter mosaic, are cost and size. It has the potential to replace many similarly constructed instruments that are widely used in traditional industries, including inspection of agricultural products, food manufacturing, military target detection, etc. It can also be used to build innovative tools or to enhance the capacity of existing ones in modern scientific research.