CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 15 > Issue 3 > Article
Open Access
1 May 2010 In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy
Zhiwei Huang, Mads Sylvest Bergholt, Wei Zheng, Kan Lin, Khek-Yu Ho, Ming The, Khay-Guan Yeoh
Author Affiliations +
Journal of Biomedical Optics, Vol. 15, Issue 3, 037017 (May 2010). https://doi.org/10.1117/1.3420115
Abstract
We first report on the implementation of a novel narrow-band image-guided Raman endoscopy technique for in vivo diagnosis of gastric dysplasia. High-quality in vivo Raman spectra can be acquired from normal and dysplastic gastric mucosal tissue within 0.5 sec under narrow-band image (NBI) guidance at gastroscopy. Significant differences are observed in in vivo Raman spectra between normal (n=54) and dysplastic (n=18) gastric tissue from 30 gastric patients, particularly in the spectral ranges of 825 to 950, 1000 to 1100, 1250 to 1500, and 1600 to 1800 cm−1, which primarily contain signals related to proteins, nucleic acids, and lipids. The multivariate analysis [i.e., principal components analysis (PCA) and linear discriminant analysis (LDA)], together with the leave-one tissue site-out, cross validation on in vivo gastric Raman spectra yields a diagnostic sensitivity of 94.4% (17/18) and specificity of 96.3% (52/54) for distinction of gastric dysplastic tissue. This study suggests that narrowband image-guided Raman endoscopy associated with PCA-LDA diagnostic algorithms has potential for the noninvasive, in vivo early diagnosis and detection of gastric precancer during clinical gastroscopic examination.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
Citation Download Citation
Zhiwei Huang, Mads Sylvest Bergholt, Wei Zheng, Kan Lin, Khek-Yu Ho, Ming The, and Khay-Guan Yeoh "In vivo early diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy," Journal of Biomedical Optics 15(3), 037017 (1 May 2010). https://doi.org/10.1117/1.3420115
Published: 1 May 2010
JOURNAL ARTICLE
 5 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 78 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Raman spectroscopy
Tissues
In vivo imaging
Endoscopy
Diagnostics
Laser tissue interaction
Proteins
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top